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Intact vector size: 5.786
Type of vector: plasmid
Cloning sites: SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS413
Host range: Saccharomyces cerevisiae, Candida robusta; Escherichia coli

Features (with orientation and position when available):
marker(s): HIS3, →, 200-1395
terminator: CYC1, →, 2077-2337
MCS: SalI...XbaI, →, 2343-2401
promoter for expression: GPD, ←, 2401-3081
marker(s): ampR, ←, 4328-5258
replicon: CEN6/ARSH4, →, 5363-5876

Vector: p413 GPD (plasmid)

Promoters: Promoter for expression GPD

Construction: pRS413

Marker(s): ampR, HIS3

Construct size (kb): 5.851

Features: marker(s): HIS3
marker(s): ampR
promoter for expression: GPD
replicon: CEN6/ARSH4
MCS: SalI...XbaI
terminator: CYC1

YX-type (expression) shuttle vector

vector with low copy number

Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.2, 0.7; EcoRI--5.9; BamHI--5.9.

Low copy number shuttle expression vector.

Temperature: 37°C

Component of: ATCC 87669