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VIII-6G10
VIII-6G10
規(guī)格:
貨期:
編號:B162279
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 VIII-6G10
商品貨號 B162279
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for VCAM-1 and blocks up to 90% of lymphocyte adhesion to microvascular endothelial cells.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with IL-4 and IL-1 beta activated endothelial cells isolated from mesenteric lymph nodes of Macaca nemestrina.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against vascular cell adhesion molecule 1 (VCAM-1, VCAM 1) of human and macaque
Cellular Products
immunoglobulin; monoclonal antibody; against vascular cell adhesion molecule 1 (VCAM-1, VCAM 1) of human and macaque
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with IL-4 and IL-1 beta activated endothelial cells isolated from mesenteric lymph nodes of Macaca nemestrina.
Spleen cells were fused with NS-1 myeloma cells.
The antibody is specific for VCAM-1 and blocks up to 90% of lymphocyte adhesion to microvascular endothelial cells.
Complete Growth Medium RPMI 1640 medium with 25 mM HEPES, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor Fred Hutchinson Cancer Res. Cntr.
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Masinovsky B, et al. IL-4 and TNF induce mAb 6G10-recognized expression on bone marrow stromal cells. US Patent 5,206,345 dated Apr 27 1993

Masinovsky B, et al. IL-4 acts synergistically with IL-1 beta to promote lymphocyte adhesion to microvascular endothelium by induction of vascular cell adhesion molecule-1. J. Immunol. 145: 2886-2895, 1990. PubMed: 1698865

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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