產(chǎn)品名稱(chēng) |
Francisella persica Larson |
商品貨號(hào) |
B162840 |
Classification |
Thiotrichales, Francisellaceae |
Deposited As |
Wolbachia persica Suitor and Weiss |
Agent |
Francisella persica Larson |
Strain |
Original |
Applications |
Vector-borne pathogens research |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1.0 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Comments |
Derived by adaptation of egg culture product ATCC® VR-331™ to Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™). ATCC® VR-331™ and ATCC® VR-1853™ have not been compared for sequence or infectivity in egg culture and tissue culture. |
Effect on Host |
CPE, refractile rounding and sloughing |
Recommended Host |
Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™) |
Growth Conditions |
Temperature: 28°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 28°C in a humidified, air atmosphere incubator. End adsorption by adding virus growth medium.
Incubation: 6-8 days at 28°C in a humidified, air atmosphere incubator, until CPE is progressed through 80% of the monolayer. |
Name of Depositor |
E Weiss, Naval Medical Research Center |
Source |
Malpighian tubules of ticks, Argas persicus (Oken) (now Argas (Persicargas) arboreus), removed from buff-backed heron (Bubulcus ibis), U.A.R. |
References |
Suitor EC, Weiss E. Isolation of a rickettsialike microorganism (Wolbachia persica n. sp.) from Argas persicus (Oken). J. Infect. Dis. 108: 95-106, 1961.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Larson MA, et al. Reclassification of Wolbachia persica as Francisella persica comb. nov. and emended description of the family Francisellaceae. Int. J. Syst. Evol. Microbiol. 66(3): 1200-1205, 2016. PubMed: 26747442
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Cross References |
Nucleotide (GenBank) :
M21292
Wolbachia persica 16S ribosomal RNA.
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