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Human Coxsackievirus A 24
Human Coxsackievirus A 24
規(guī)格:
貨期:
編號(hào):B163339
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Human Coxsackievirus A 24
商品貨號(hào) B163339
Classification Picornaviridae, Enterovirus, Human enterovirus C
Agent Human Coxsackievirus A 24
Strain DN-19
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
This item is a mycoplasma free preparation derived from VR-583 following treatment to remove mycoplasma. VR-583 will no longer be distributed when current inventory is depleted. Strain DN-19 is considered to be an antigenic variant or "prime" of Human coxsackievirus A 24. It was originally known as Echovirus 34.
Product Format frozen
Storage Conditions -70°C or colder
Comments
This item is a mycoplasma free preparation derived from VR-583 following treatment to remove mycoplasma. VR-583 will no longer be distributed when current inventory is depleted. Strain DN-19 is considered to be an antigenic variant or "prime" of Human coxsackievirus A 24. It was originally known as Echovirus 34.
Effect on Host
CPE in culture include highly refractile, rounded cells throughout monolayer or in loose clusters
CPE in culture include highly refractile, rounded cells throughout monolayer or in loose clusters.
Growth Medium ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host: WI-38 (ATCC CCL-75)

Recommended Host: ?WI-38 cells (ATCC? CCL-75)

Alternate Hosts: Human embryonic kidney cells, human aorta cells (A-39)
Growth Conditions
For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluence using a multiplicity of infection (MOI) of 0.1 to 1.0. Incubate at 37.0°C in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired.
Name of Depositor HA Wenner
Source
Isolated from infant with diarrhea, Texas, 1963; derived from ATCC VR-583 following treatment to remove mycoplasma contamination.
References

The Work of WHO Virus Reference Centers and the Services they Provide, Geneva. Bull. World Health Organ 11: 1968.

Schmidt NJ, Lennette EH. Antigenic variants of coxsackievirus type A24. Am. J. Epidemiol. 91: 99-109, 1970. PubMed: 4313417

Rosen L et al. Subclassification of enteroviruses and ECHO virus type 34. Brief report. Archives of Virology 30: 89-92, 1970. PubMed: 4986680.

Brown BA. Resolving ambiguities in genetic typing of human enterovirus species C clinical isolates and identification of enterovirus 96, 99 and 102. J. Gen. Virol. 90 (PT 7): 1713-1723, 2009. PubMed: 19264596

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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