国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 15F7
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
15F7
15F7
規(guī)格:
貨期:
編號:B163587
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 15F7
商品貨號 B163587
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties mixed: adherent and suspension, The cells are rather adherent in nature, and grow best if maintained in suspension culture plates (e.g., Corning Catalog number 430591).
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain Wistar (B cell); BALB/c (myeloma)
Applications
The antibody reacts with CD1.1, but cross-reacts with CD1.2 only at very high concentrations.
The antibody performs well in flow cytometry applications, and is also useful in immunoprecipitation applications.
Derivation
Spleen cells were fused with Sp2/0-Ag14 cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD1.1
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CD1.1
Comments
Animals were immunized with C57BL/6 thymocytes, boosted three times with the RBL-CD1.1 rat cell line (CD1.1-transfected), boosted once with BALB/cJ thymocytes and finally with the CR1-CD1.1 human cell line (CD1.1-transfected).
Spleen cells were fused with Sp2/0-Ag14 cells.
The antibody reacts with CD1.1, but cross-reacts with CD1.2 only at very high concentrations.
The antibody blocks recognition of CD1.1 by T cells in vitro.
The antibody performs well in flow cytometry applications, and is also useful in immunoprecipitation applications.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 95%; fetal bovine serum, 5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Maintain cultures at cell concentrations between 2 X 10(5) and 8 X 10(5) viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2b; kappa light chain
Name of Depositor A Bendelac
Deposited As rat (B cell); mouse (myeloma)
References

Roark JH, et al. CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells. J. Immunol. 160: 3121-3127, 1998. PubMed: 9531266

Park SH, et al. Tissue-specific recognition of mouse CD1 molecules. J. Immunol. 160: 3128-3134, 1998. PubMed: 9531267

Park SH, et al. Innate and adaptive functions of the CD1 pathway of antigen presentation. Semin. Immunol. 10: 391-398, 1998. PubMed: 9799714

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
边坝县| 汨罗市| 太原市| 阿克苏市| 诸城市| 瑞昌市| 太仓市| 长顺县| 高州市| 临桂县| 巴彦县| 金乡县| 乌什县| 武宣县| 高碑店市| 绵阳市| 扎鲁特旗| 榆树市| 巴中市| 阜康市| 清原| 海盐县| 积石山| 三门峡市| 平原县| 毕节市| 梓潼县| 横山县| 乌海市| 齐齐哈尔市| 大同市| 嘉荫县| 原平市| 青川县| 乌海市| 修武县| 金山区| 浪卡子县| 奈曼旗| 应城市| 乌恰县|