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803-15.6
803-15.6
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貨期:
編號(hào):B163834
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 803-15.6
商品貨號(hào) B163834
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with both native and denatured HIV-1 gp120 within the conserved C-terminal amino acid sequence residues 495-516.
The antibody can be used for the purification of gp120 using a milder elution condition (pH4) than many other antibodies; it can be used in ELISA and Western Blot assays.
Storage Conditions liquid nitrgoen vapor phase
Derivation
Animals were immunized with recombinant immunodeficiency virus 1 (HIV-1) gp120 (IIIB variant) purified from Baculovirus supernatant.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120.
Cellular Products
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120.
Comments
Animals were immunized with recombinant immunodeficiency virus 1 (HIV-1) gp120 (IIIB variant) purified from Baculovirus supernatant.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The cell line was subcloned six times and adapted to growth in serum-free medium.
The antibody reacts with both native and denatured HIV-1 gp120 within the conserved C-terminal amino acid sequence residues 495-516.
The antibody can be used for the purification of gp120 using a milder elution condition (pH4) than many other antibodies; it can be used in ELISA and Western Blot assays.
Complete Growth Medium HB101 medium supplemented with 2 mM L-glutamine, 1.0 mM sodium pyruvate, 10 mM HEPES and 0.021 mM 2-mercaptoethanol
Subculturing Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and  1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor MR van Schravendijk
Deposited As mouse (B cell); mouse (myeloma)
References

Golden A, et al. Effect of promoters and signal sequences on the production of secreted HIV-1 gp120 protein in the baculovirus system. Protein Expr. Purif. 14: 8-12, 1998. PubMed: 9758745

Ferrer M, et al. Construction and characterization of a radio-iodinatable mutant of recombinant human CD4. J. Immunol. Methods 210: 215-225, 1997. PubMed: 9520304

Ferrer M, Harrison SC. Peptide ligands to human immunodeficiency virus type 1 gp120 identified from phage display libraries. J. Virol. 73: 5795-5802, 1999. PubMed: 10364331

Ferrer M, et al. Structural and functional characterization of an epitope in the conserved C-terminal region of HIV-1 gp120. J. Peptide Res. 54: 32-42, 1999. PubMed: 10448968

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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