国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > CCD-1094Sk
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
CCD-1094Sk
CCD-1094Sk
規(guī)格:
貨期:
編號(hào):B164165
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 CCD-1094Sk
商品貨號(hào) B164165
Organism Homo sapiens, human
Tissue skin
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Carcinoma
Age 57 years
Gender female
Ethnicity Caucasian
Applications
The line was established from normal skin taken at biopsy from the left breast of a patient with metastatic mammary carcinoma.
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was established from normal skin taken at biopsy from the left breast of a patient with metastatic mammary carcinoma.
Clinical Data
The line was established from normal skin taken at biopsy from the left breast of a patient with metastatic mammary carcinoma.
female
Caucasian
57 years
Comments
The line was established from normal skin taken at biopsy from the left breast of a patient with metastatic mammary carcinoma.
The cells senesce after approximately 41 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25%Trypsin-0.53mM EDTA solution.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:3 to 1:6
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
温宿县| 兴业县| 绥宁县| 南和县| 秀山| 镇巴县| 仁化县| 额尔古纳市| 长白| 白水县| 灵寿县| 孝昌县| 商城县| 五常市| 西宁市| 吕梁市| 宁海县| 周宁县| 乌兰县| 兰坪| 聊城市| 肥东县| 漯河市| 长白| 嘉定区| 定远县| 峡江县| 龙南县| 平乡县| 麦盖提县| 咸宁市| 淄博市| 乐陵市| 新蔡县| 济阳县| 灵石县| 宾川县| 定州市| 皋兰县| 云霄县| 鹤山市|