国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > J.CaM1.6 (derivative mutant of Jurkat)
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
J.CaM1.6 (derivative mutant of Jurkat)
J.CaM1.6 (derivative mutant of Jurkat)
規(guī)格:
貨期:
編號:B164866
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 J.CaM1.6 (derivative mutant of Jurkat)
商品貨號 B164866
Organism Homo sapiens, human
Tissue Peripheral blood
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease acute T cell leukemia
Gender male
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Derivation
The J.CaM1.6 cell line is a derivative mutant of Jurkat. The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM. The J.CaM1.6 line was derived from the E6-1 clone of Jurkat (ATCC TIB-152) by treatment with ethylmethanesulfonate (EMS). Cells were then selected for T cell antigen receptor (TCR) expression and inability to increase intracellular calcium in response to TCR stimulation with OKT3 antibody.
Clinical Data
male
14 years
Comments CD3/T1 complexes are present; however, TCR signal transduction is defective. They are deficient in lck kinase activity and are missing exon 7 in their lck mRNA. The T cell antigen receptor appears to be structurally normal.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL. Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL.
Medium Renewal: Twice per week
Cryopreservation
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 10,11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18,19
Name of Depositor A Weiss
Deposited As Homo sapiens
References

Straus DB, Weiss A. Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor. Cell 70: 585-593, 1992. PubMed: 1505025

Goldsmith MA, Weiss A. Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor. Proc. Natl. Acad. Sci. USA 84: 6879-6883, 1987. PubMed: 3309950

Lund T, et al. Herpesvirus saimiri Tip-484 membrane protein markedly increases p56lck activity in T cells. J. Virol. 71: 378-382, 1997. PubMed: 8985360

Cenciarelli C, et al. T cell antigen receptor ubiquitination is a consequence of receptor-mediated tyrosine kinase activation. J. Biol. Chem. 271: 8709-8713, 1996. PubMed: 8621503

Gibson S, et al. Functional LCK is required for optimal CD28-mediated activation of the TEC family tyrosine kinase EMT/ITK. J. Biol. Chem. 271: 7079-7083, 1996. PubMed: 8636141

Hutchcroft JE, et al. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J. Biol. Chem. 271: 13362-13370, 1996. PubMed: 8662792

Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013

Ronald Wange, personal communication

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
乌苏市| 库车县| 锦州市| 正定县| 合作市| 清新县| 肃宁县| 仁寿县| 胶州市| 大洼县| 天津市| 延庆县| 德江县| 彰化市| 修水县| 思茅市| 通山县| 犍为县| 甘孜县| 巧家县| 城步| 农安县| 滨海县| 吉木乃县| 收藏| 宜黄县| 嘉定区| 漳平市| 怀柔区| 贵定县| 江阴市| 山丹县| 衡水市| 邹平县| 陈巴尔虎旗| 麟游县| 称多县| 策勒县| 连州市| 陇南市| 视频|