| 產(chǎn)品名稱 |
KHOS-312H |
| 商品貨號 |
B164913 |
| Organism |
Homo sapiens, human |
| Tissue |
bone |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
osteosarcoma |
| Age |
13 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Clinical Data |
female Caucasian 13 years |
| Tumorigenic |
No |
| Effects |
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments |
The growth properties of KHOS-312H are similar to HOS (ATCC CRL-1543).
This line is a revertant of ATCC CRL-1544 that no longer has a rescuable Kirsten murine sarcoma virus genome.
Note: An inconsistency in our records was discovered late in 2010 concerning the human osteosarcoma cell line, KHOS-312H. Due to an ATCC catalog transfer error, this cell line has been referred to as both KHOS-312H and KHOS-321H by ATCC and in the literature. The name originally supplied to ATCC by the depositor was KHOS-312H. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, rinse with fresh 0.25% trypsin-0.53 mM EDTAsolution, remove trypsin and let the culture sit at 37°C for 10 to 12 minutes or until cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 days.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: 2 to 3 times per week
|
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 THO1: 6 TPOX: 8,11 vWA: 18 |
| Name of Depositor |
JS Rhim |
| Deposited As |
Homo sapiens |
| References |
Yang YH, et al. Reversion of Kirsten sarcoma virus transformed human cells: elimination of the sarcoma virus nucleotide sequences. J. Gen. Virol. 43: 447-451, 1979. PubMed: 225428
Cho HY, et al. Revertants of human cells transformed by murine sarcoma virus. Science 194: 951-953, 1976. PubMed: 62397
Cho HY, Rhim JS. Cycloheximide-dependent reversion of human cells transformed by MSV and chemical carcinogen. Science 205: 691-693, 1979. PubMed: 223242
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