国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門(mén)搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車(chē) 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > KM114
最近瀏覽歷史
更多產(chǎn)品
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
KM114
KM114
規(guī)格:
貨期:
編號(hào):B164919
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) KM114
商品貨號(hào) B164919
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with the BMS2 bone marrow derived stromal cell line.
KM114 recognizes an epitope close to, but distinct from, those detected by KM201 and KM81 (see ATCC TIB-240 and ATCC TIB-241).
Derivation
Animals were immunized with the BMS2 bone marrow derived stromal cell line.
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the functional domain of mouse Pgp-1 (CD44)
Cellular Products
immunoglobulin; monoclonal antibody; against the functional domain of mouse Pgp-1 (CD44)
Comments
Animals were immunized with the BMS2 bone marrow derived stromal cell line.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody blocks adhesion of a B cell hybridoma (BM-2) to BMS2 cells or to hyaluronate coated dishes.
The antibody prevents growth of lymphoid and myeloid cells in long term bone marrow cultures.
KM114 recognizes an epitope close to, but distinct from, those detected by KM201 and KM81 (see ATCC TIB-240 and ATCC TIB-241).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor PW Kincade
Deposited As rat (B cell); mouse (myeloma)
References

Miyake K, et al. Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures. J. Exp. Med. 171: 477-488, 1990. PubMed: 2406365

Miyake K, et al. Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition. J. Exp. Med. 172: 69-75, 1990. PubMed: 2193100

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
九江市| 天水市| 荣成市| 柳州市| 孙吴县| 温宿县| 景东| 延边| 昌图县| 柳州市| 康平县| 甘德县| 永善县| 弥渡县| 辽中县| 新绛县| 阿拉善右旗| 巴林右旗| 武功县| 云林县| 吉林省| 二手房| 田阳县| 精河县| 辽阳县| 安丘市| 宁安市| 建湖县| 普洱| 丹凤县| 克山县| 宝应县| 贵州省| 斗六市| 苗栗县| 吴忠市| 绿春县| 临邑县| 苏尼特左旗| 双鸭山市| 安塞县|