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MARC S5
MARC S5
規(guī)格:
貨期:
編號:B165082
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 MARC S5
商品貨號 B165082
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for E. coli O157 O-antigen and for the Salmonella serogroup N O-antigen (which is identical to the E. coli O157 antigen).
High titer ascites can be produced but these cells become lethal to mice within approximately 5 days post-intraperitioneal injection.
The antibody has been used in ELISA, Western Blot, lateral flow and chemiluminescense assays.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Both ascites and culture media react with extremely high affinity. High titer ascites can be produced but these cells become lethal to mice within approximately 5 days post-intraperitioneal injection.
Genes Expressed
immunoglobulin; monoclonal antibody; against E. coli O157 O-antigen
Cellular Products
immunoglobulin; monoclonal antibody; against E. coli O157 O-antigen
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with whole bacterium antigen from E. coli O157:H7 (ATCC 43895).
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody is specific for E. coli O157 O-antigen and for the Salmonella serogroup N O-antigen (which is identical to the E. coli O157 antigen).
Both ascites and culture media react with extremely high affinity. High titer ascites can be produced but these cells become lethal to mice within approximately 5 days post-intraperitioneal injection.
The antibody has been used in ELISA, Western Blot, lateral flow and chemiluminescense assays.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM nonessential amino acids, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.

Medium Renewal:  Add fresh medium every 2 to 3 days (depending on cell density)

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgM
Name of Depositor JE Keen
Deposited As mouse (B cell); mouse (myeloma)
References

Westerman RB, et al. Production and characterization of monoclonal antibodies specific for the lipopolysaccharide of Escherichia coli O157. J. Clin. Microbiol. 35: 679-684, 1997. PubMed: 9041412

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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