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MV-4-11
MV-4-11
規(guī)格:
貨期:
編號(hào):B165206
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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RNA

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凍干粉
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產(chǎn)品名稱 MV-4-11
商品貨號(hào) B165206
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type macrophage
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease biphenotypic B myelomonocytic leukemia
Age 10 years
Gender male
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype 48, XY, t(4;11)(q21;q23), +8, +19
Derivation
The MV4-11 cell line was established by Rovera and associates from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia. The growth factor, granulocyte/macrophage colony-stimulating factor (GM-CSF), was required to establish this cell line and growth factors are necessary for its continuous proliferation in chemically defined medium [PubMed: 3496132].
Clinical Data
male
The MV4-11 cell line was established by Rovera and associates from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia.
Antigen Expression
CD4; Homo sapiens
CD10; Homo sapiens
CD15, human; Homo sapiens
CD4 (40-96%); CD10 (4-11%); CD15 (96-99%)
Genes Expressed
CD4; Homo sapiens, CD10; Homo sapiens, CD15, human; Homo sapiens, CD4 (40-96%); CD10 (4-11%); CD15 (96-99%)
Comments
This line can be propagated in medium supplemented with 10% FBS without the addition of growth factors. IL-3 can independently support the long-term growth of this cell line, but IL-3 antagonized the proliferation of MV4-11 cells in the presence of GM-CSF when both factors were used at very low concentrations. Granulocyte colony stimulating factor (G-CSF) synergized with GM-CSF in inducing proliferation of MV4-11 cells; G-CSF alone causes a transient stimulation of the cell line.
This line was formerly designated ATCC HTB-189.
Over 96% of these cells are positive by indirect immunofluorescence for the myelomonocytic antigen CD15, 40-96% are positive for the monocytic antigen CD4, and 4-11% are positive for CD10 [PubMed: 3500218].
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Every 2 to 3 days
Note: If the cells are maintained in a serum-free medium, it is necessary to add the following: 0.005 mg/mL transferrin, 0.005 mg/mL insulin, and 5 ng/mL GM-CSF.
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 8,9.3
TPOX: 8,11
vWA: 14,15
Name of Depositor Wistar Institute
Deposited As Homo sapiens
U.S. Patent Number
References

Lange B, et al. Growth factor requirements of childhood acute leukemia: establishment of GM-CSF-dependent cell lines. Blood 70: 192-199, 1987. PubMed: 3496132

Santoli D, et al. Synergistic and antagonistic effects of recombinant human interleukin (IL) 3, IL-1 alpha, granulocyte and macrophage colony-stimulating factors (G-CSF and M-CSF) on the growth of GM-CSF-dependent leukemic cell lines. J. Immunol. 139: 3348-3354, 1987. PubMed: 3500218

If the cells are maintained in a serum-free medium, it is necessary to add the following: 0.005 mg/ml transferrin, 0.005 mg/ml insulin, and 5 ng/ml GM-CSF.

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