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MYC CT 9-B7.3
MYC CT 9-B7.3
規(guī)格:
貨期:
編號:B165215
品牌:Mingzhoubio

標準菌株
定量菌液
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RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 MYC CT 9-B7.3
商品貨號 B165215
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human myc (c-myc) protein
Cellular Products
immunoglobulin; monoclonal antibody; against human myc (c-myc) protein
Comments
Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody precipitates the p62 c-myc protein, but does not react with mouse or chicken c-myc.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor J Bishop, GI Evan
Deposited As mouse (B cell); mouse (myeloma)
References

Evan GI, et al. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5: 3610-3616, 1985. PubMed: 3915782

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于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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