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NAMALWA
NAMALWA
規(guī)格:
貨期:
編號:B165224
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 NAMALWA
商品貨號 B165224
Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2

[Cells contain Herpesvirus]


Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Burkitt's lymphoma
Age 3 years
Gender female
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a human cell line with the hypodiploid chromosome count. The modal chromosome number was 44, occurring in 30% of cells. The rate of cells with higher ploidies was 2.8%. Twelve to 14 marker chromosomes were common to most cells. Among these were ins(1;?) (q11;?), ins(3) (q11q29), del(3) (p12/13), der(6)t(3;6) (p21;p25), t(15q21q) and seven to nine others. Normal N3 was absent, and N7 had three copies per cell. The X chromosome was single. There was no Y chromosome.
Images CRL-1432 Micrograph
Clinical Data
female
3 years
Genes Expressed
immunoglobulin
Comments
The cells contain the Epstein-Barr virus (EBV) genome.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 92.5%; fetal bovine serum, 7.5%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Cultures should be started at 5 x 105 viable cells/mL and subcultured at 2 x 106 cells/mL.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 7,9.3
TPOX: 6,11
vWA: 14
Isotype IgM; lambda light chain
Name of Depositor NB Finter
Deposited As Homo sapiens
References

Klein F, et al. Large-scale production and concentration of human lymphoid interferon. Antimicrob. Agents Chemother. 15: 420-427, 1979. PubMed: 464569

Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763

Klein G, et al. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus. Int. J. Cancer 10: 44-57, 1972. PubMed: 4122458

Nyormoi O, et al. Sensitivity to EBV superinfection and IUdR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line. Int. J. Cancer 12: 396-408, 1973. PubMed: 4365093

Cross References

Nucleotide (GenBank) : X59969 H.sapiens BiP/GRP78 promoter.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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