国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > NCI-H647 [H647]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
NCI-H647 [H647]
NCI-H647 [H647]
規(guī)格:
貨期:
編號(hào):B165354
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 NCI-H647 [H647]
商品貨號(hào) B165354
Organism Homo sapiens, human
Tissue lung; derived from metastatic site: pleural effusion
Product Format frozen
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease stage 3A, adenosquamous carcinoma; non-small cell lung cancer
Age 56 years
Gender male
Ethnicity Caucasian
Karyotype modal number = 89; range = 74-176; t(3p14;11p15)
Derivation
The line was established in October 1983.
Clinical Data
56 years
Caucasian
male
The patient received prior radiation therapy.
The tissue donor was a non-smoker.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. 
Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 9,11
D16S539: 9
D5S818: 12
D7S820: 10
THO1: 6,9.3
TPOX: 11
vWA: 17
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
Year of Origin 1983
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
平昌县| 正阳县| 惠东县| 德庆县| 邓州市| 米泉市| 洛扎县| 宁晋县| 东至县| 松阳县| 昭通市| 罗江县| 冕宁县| 葵青区| 雷州市| 徐汇区| 平顶山市| 巴彦县| 徐汇区| 汕头市| 武邑县| 柞水县| 九台市| 延川县| 清原| 丽江市| 施甸县| 巨野县| 江川县| 铁岭市| 富民县| 铜山县| 尚义县| 南溪县| 平泉县| 墨玉县| 赞皇县| 桑植县| 文山县| 包头市| 武陟县|