国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > WEHI-3
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
WEHI-3
WEHI-3
規(guī)格:
貨期:
編號(hào):B165944
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 WEHI-3
商品貨號(hào) B165944
Organism Mus musculus, mouse
Tissue peripheral blood
Product Format frozen
Culture Properties suspension (some adherent cells)
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease leukemia
Strain BALB/c
Applications
Tested and found negative for ectromelia virus (mousepox).
The cells exhibit only weak effector activity in antibody dependent cell mediated cytotoxicity.
Storage Conditions liquid nitrogen vapor phase
Receptor Expression
complement (C3)
Genes Expressed
lysozyme; granulocyte colony stimulating activity (CSA); interleukin-3 (interleukin 3, IL-3)
Cellular Products
lysozyme; granulocyte colony stimulating activity (CSA); interleukin-3 (interleukin 3, IL-3)
Comments
The growth of WEHI-3 is inhibited by 4 ng/mL LPS and blocked by higher concentrations.
Dextran sulfate at 30 to 40 µg/mL also inhibits growth.
Latex beads are phagocytized but are not toxic.
Zymosan and BCG are phagocytized and block growth.
The cells exhibit only weak effector activity in antibody dependent cell mediated cytotoxicity.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • fetal bovine serum to a final concentration of 10%
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL.
Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL. Adherent cells may be harvested by scraping.

Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor M Cohn
Deposited As Mus musculus
References

Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890

Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

McKean DJ, et al. Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells. J. Exp. Med. 154: 1419-1431, 1981. PubMed: 6170720

Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
郁南县| 竹北市| 金华市| 双鸭山市| 文登市| 阿巴嘎旗| 怀安县| 岳阳市| 城固县| 哈尔滨市| 清流县| 荣成市| 博客| 象州县| 延寿县| 民权县| 华阴市| 璧山县| 永定县| 昂仁县| 阳泉市| 韶山市| 油尖旺区| 宝鸡市| 尼木县| 辰溪县| 芮城县| 株洲县| 哈密市| 曲阳县| 平邑县| 安龙县| 英吉沙县| 诏安县| 青浦区| 衡阳市| 白水县| 开化县| 顺平县| 琼海市| 天柱县|