| 產(chǎn)品名稱 |
XMMME-001 |
| 商品貨號(hào) |
B165972 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
When cultures are seeded at 5 X 10(4) cells/ml, antibody production may not be detectable until days 6 or 7. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human melanoma tumor specific antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human melanoma tumor specific antigen |
| Comments |
When cultures are seeded at 5 X 10(4) cells/ml, antibody production may not be detectable until days 6 or 7. Animals were immunized with M21 human melanoma cells. Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10(4) cells/ml and maintain between 1 X 10(5) and 2 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Isotype |
IgG2a |
| Name of Depositor |
Xoma Corporation |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Scannon PJ, et al. Human melanoma specific immunotoxins. US Patent 4,590,071 dated May 20 1986
Levy JA, et al. Purification of monoclonal antibodies from mouse ascites eliminates contaminating infectious mouse type C viruses and nucleic acids. Clin. Exp. Immunol. 56: 114-120, 1984. PubMed: 6201307
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