產(chǎn)品名稱 |
SU-DHL-10 |
商品貨號(hào) |
B167194 |
Organism |
Homo sapiens, human |
Tissue |
lymph node
Derived from Metastatic Site: peritoneal effusion |
Cell Type |
B lymphocyte |
Product Format |
frozen |
Morphology |
lymphoblast-like |
Culture Properties |
suspension, multicellular aggregates |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
Large Cell Lymphoma |
Age |
25 years |
Gender |
Male |
Ethnicity |
Caucasian |
Storage Conditions |
liquid nitrogen vapor phase |
Images |
![CRL-2963 Micrograph](/~/media/Attachments/Micrographs/Cell/CRL-2963.ashx) |
Clinical Data |
25 years
Caucasian
Male |
Comments |
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR. |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
Subculturing |
Cultures can be maintained by the addition of fresh medium. An inoculum of 8.0 x 104 to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 1.0 x 106 and 2.0 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:12 is recommended.
Medium renewal: every 2 to 4 days |
Cryopreservation |
Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase |
Culture Conditions |
Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
STR Profile |
D5S818: 11, 12
D13S317: 10, 11
D7S820: 8, 11
D16S539: 10, 11
vWA: 17
THO1: 8, 9.3
TPOX: 8
CSF1PO: 10, 13
Amelogenin: XY |
Isotype |
IgG lambda |
Name of Depositor |
A Epstein |
Year of Origin |
1978 |