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Paramecium dodecaurelia Sonneborn
Paramecium dodecaurelia Sonneborn
規(guī)格:
貨期:
編號:B219855
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Paramecium dodecaurelia Sonneborn
商品貨號 B219855
Strain Designations stock 246.23
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Red Creek, Pearl River Co., MS, 1956
Product Format test tube
Storage Conditions Test Tube: See handling procedure
Axenic/Xenic Xenic
Type Strain yes
Comments
species description
species description
Medium ATCC® Medium 802: Sonneborn's Paramecium medium
Growth Conditions
Temperature: 25°C
Cryopreservation

Cryoprotective Solution
DMSO, 1.5 ml
Fresh growth medium w/o bacteria, 7.5 ml
MgCl2 (0.5 mM), 0.5 ml
CaCl2 (0.5 mM), 0.5 ml

  1. Mix the components in the order listed.  Before adding the MgCl2 and the CaCl2 allow the solution to return to room temperature.  When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 200 x g for 1 min.
  3. Adjust the concentration of cells to 2 x 105/ml in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state add 1.0 ml ATCC medium 802 to the frozen ampule and place it in a 35°C water bath.  Immerse the vial  to a level just above the surface of the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate onto the surface of an ATCC medium 919 (non-nutrient agar) plate containing an overlay of 15.0 ml of bacterized ATCC medium 802.
  10. Incubate at 25°C.
  11. Once the culture is established, transfer 0.5 ml to 5.0 ml of bacterized ATCC medium 802.
  12. Follow the protocol for maintenance of culture.
Name of Depositor TM Sonneborn
Chain of Custody
ATCC <-- TM Sonneborn <-- L. Gilman
Year of Origin 1956
References

Aufderheide KJ. Paramecium sonneborni n. sp. new member of the Paramecium aurelia species-complex. J. Protozool. 30: 128-131, 1983.

Sonneborn TM. The Paramecium aurelia complex of fourteen sibling species. Trans. Am. Microsc. Soc. 94: 155-178, 1975.

. The biology of Paramecium, 2nd ed. New York: Plenum Press; 1986.

type strain

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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