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p416 GAL1 in E. coli
p416 GAL1 in E. coli
規(guī)格:
貨期:
編號(hào):B230066
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) p416 GAL1 in E. coli
商品貨號(hào) B230066
Designations p416 GAL1 in E. coli
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 5.5840001106262210
Vector: p416 GAL1 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pRS416, GAL1 promoter
Marker(s):URA3,ampR
Construct size (kb): 5.584000110626221
Features: marker(s): URA3
marker(s): ampR
promoter for expression: GAL1
MCS: XhoI...XbaI
terminator: CYC1
centromere: CEN6/ARSH4
Applications
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.1, 0.5; EcoRI--5.6; KpnI--5.6.
Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

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