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pHP45omegavph
pHP45omegavph
規(guī)格:
貨期:
編號:B234465

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pHP45omegavph
商品貨號 B234465
Designations pHP45omegavph
Depositors J Pernodet, M Blondelet-Rouault
Biosafety Level 1
Vector Information
Construct size (kb): 4.916999816894531
Applications
integrating vector
shuttle vector
vector permitting positive selection for integration
vector useful for interposon mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): ClaI--5.0; EcoRI--2.6, 2.3; SphI--3.3, 0.9, 0.8.
One of three vectors (ATCC 87626 - 87628) containing antibiotic resistance gene cassettes, derived from the omega interposon. The cassettes can be used for insertional mutagenesis or for vector construction.
Antibiotic concentration used for selection on standard media, e.g. Nutrient Agar for E. coli and R2YE for Streptomyces lividans is 30 ug/ml viomycin (Vm).
After transformation of competent cells, 3 to 4 hours without selection were required for expression of the cassette, before the cells could be plated on selective medium.
After transformation of comopetent cells, 3 to 4 hours without selection were required for expression of the cassette, before the cells could be plated on selective medium.
It is sometimes easier to select transformed E. coli using another selection marker, and to subsequently select the ones having received omega-vph by replica plating on medium with viomycin.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Blondelet-Rouault MH, et al. Antibiotic resistance gene cassettes derived from the omega interposon for use in E. coli and Streptomyces. Gene 190: 315-317, 1997. PubMed: 9197550

Jean-Luc Pernodet, personal communication

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