| 產(chǎn)品名稱 |
Sp2/mIL-6 |
| 商品貨號(hào) |
B165750 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
Hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
Lymphoblast-like |
| Culture Properties |
Suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
It is a mouse fusion cell line derived from Sp2/0 cells that had been transfected with high-titer L6PNL recombinant retrovirus carrying a mouse interleukin-6 (IL-6) cDNA under the transcriptional control of the viral LTR and the neo gene encoding bacterial neomycin phosphotransferase (G418 resistance).
Sp2/IL-6 cells secrete IL-6 and are an improved myeloma fusion parent in terms of both the relative number of viable B cell hybridomas producing immunoglobulin and the proportion of hybridomas producing antigen-specific monoclonal antibodies.
Sp2/IL-6 cells secrete IL-6 and are an improved myeloma fusion parent in terms of both the relative number of viable B cell hybridomas producing immunoglobulin and the proportion of hybridomas producing antigen-specific monoclonal antibodies.
Sp2/mIL-6 cells have stably incorporated in their genome approximately six copies of the retroviral vector L6PNL and constitutively secrete large quantities of mouse IL-6 (10,000 units/ml).
|
| Derivation |
The Sp2/mIL-6 line was established by R. Hawley and T. Hawley of the Sunnybrook Health Centre, Toronto, Canada. It is a mouse fusion cell line derived from Sp2/0 cells that had been transfected with high-titer L6PNL recombinant retrovirus carrying a mouse interleukin-6 (IL-6) cDNA under the transcriptional control of the viral LTR and the neo gene encoding bacterial neomycin phosphotransferase (G418 resistance). |
| Antigen Expression |
Antigen expression: H-2, d haplotype; Mus musculus, expressed |
| Genes Expressed |
mouse interleukin 6 (interleukin-6, IL-6),H-2, d haplotype; Mus musculus, expressed |
| Cellular Products |
mouse interleukin 6 (interleukin-6, IL-6) |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
- 0.05 mM 2-Mercaptoethanol
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 2 X 10(5) cells/ml and subculture before the cell density reaches 2 X 10(6) cells/ml. Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density) |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
JF Harris |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Harris JF, et al. Increased frequency of both total and specific monoclonal antibody producing hybridomas using a fusion partner that constitutively expresses recombinant IL-6. J. Immunol. Methods 148: 199-207, 1992. PubMed: 1373425
|
