| 產(chǎn)品名稱 |
SR |
| 商品貨號(hào) |
B165751 |
| Organism |
Homo sapiens, human |
| Tissue |
pleural effusion |
| Cell Type |
lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
large cell immunoblastic lymphoma |
| Age |
11 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
SR is a human lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo. |
| Clinical Data |
11 years Caucasian male |
| Antigen Expression |
Hle-1 +; HLA DQ +; HLA DR +; CD25 +; CD19 -; CD20 -; CD21 -; CD22 -; T cell receptor (TCR) - |
| Comments |
SR is of undetermined cellular origin because it expresses no markers unique to B, T, natural killer (NK) or monocyte-lineages. Exposure of SR cells to protein kinase C activating phorbol esters such as PMA and PdBu do not induce growth inhibition. SR cells have been reported to be Epstein-Barr virus genome negative. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 - 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: 2 to 3 times a week. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| STR Profile |
Amelogenin: X,Y CSF1PO: 14,15 D13S317: 11 D16S539: 11,13 D5S818: 11 D7S820: 9,12 THO1: 6,7 TPOX: 8,11 vWA: 17,18 |
| Name of Depositor |
M Beckwith |
| Deposited As |
Homo sapiens |
| References |
Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723
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