Quantitative Synthetic Saint Louis encephalitis virus RNA
商品貨號(hào)
B206016
Agent
Quantitative Synthetic Saint Louis encephalitis virus RNA
Applications
ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen Specification range: 1 x 105 to 1 x 106 copies/µL 100 μL per vial with Biomatrica RNAstable
Storage Conditions
-70°C or colder
Intended Use
The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.
Comments
Manufactured under ISO 13485 guidance
Preparation includes fragments from pre-membrane, envelope, and nonstructural genes NS1, NS5, and 3’UTR.
The following primers and probe can be used with this nucleic acid preparation RefLanciotti RS, Kerst AG. Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses. J. Clin. Microbiol. 39(12): 4506-4513, 2001. PubMed: 11724870:
Forward primer: CTGGCTGTCGGAGGGATTCT
Reverse primer: TAGGTCAATTGCACATCCCG
Probe: FAM-TCTGGCGACCAGCGTGCAAGCCG-BHQ
Special Collection
RNA
References
Lanciotti RS, Kerst AG. Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses. J. Clin. Microbiol. 39(12): 4506-4513, 2001. PubMed: 11724870