Comments |
Restriction digests of the clone give the following sizes (kb): XhoI--3.0, 2.0; PstI--2.55, 1.25, 0.76, 0.5; HindIII--5.2; BamHI--3.2, 1.3, 0.62; ClaI--3.7, 0.93, 0.46. This is identical to pHUG86.1. When excised with XhoI, some pcD sequence is retained. |