Restriction digests of the clone give the following sizes (kb): HindIII--4.3, 2.6; EcoRI--6.2, 0.6; PvuII--6.9; BamHI--4.3, 2.6; PstI--6.9. Detects genomic BssHII fragments of the following sizes (kb): 600, 230, 80. Detects the following genomic restriction fragments (kb): SacII--560, 160; SfiI--700; SalI--420, 350. Subcloned from cosmid cNX.6d. |