Insert contains the following sites separated by (bp)(approx): HindIII- 195- PvuII- 780- AccI- 350- PstI. Restriction digests of the clone give the following sizes (kb): HindIII--4.0; PstI--4.0; HindIII/PstI--2.7, 1.35. The insert spans nucleotides 5080-6395 of TMV. |