Comments |
Restriction digests of the clone give the following sizes (kb): PstI--23.0, 7.5, 2.9, 2.5, 2.1, 1.79, 1.22, 1.15, 0.84, 0.53, 0.49, 0.46; HindIII--23.0, 21.0, 3.3, 2.4; EcoRI--23.0 (doublet), 5.4; BglI--23.0, 6.8, 6.2, 3.8, 3.5, 2.7, 1.9, 0.96; BamHI--23.0, 20.0, 5.9, 2.9, 1.68, 1.49. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HincII, MspI, PstI, RsaI, TaqI (panel of 5 individuals). |