(Aromatic residues of RRM1, RRM2, RRM3 and RRM4 have been mutated)
HIS(6) tag added to the amino terminus.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Deardorff JA. Differential effects of aromatic and charged residue substitutions in the RNA binding domains of the yeast poly(A)-binding protein.. J. Mol. Biol. 269: 67-81, 1997. PubMed: 9193001