国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Microvascular Endothelial Cell Growth Kit-VEGF
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Microvascular Endothelial Cell Growth Kit-VEGF
Microvascular Endothelial Cell Growth Kit-VEGF
規(guī)格:
貨期:
編號:B208382
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


<fieldset id="wzyfx"><del id="wzyfx"><sup id="wzyfx"></sup></del></fieldset><div id="wzyfx"></div>
      2. 
  
    
      
      產(chǎn)品名稱
      Microvascular Endothelial Cell Growth Kit-VEGF
    
      
    
      
      商品貨號
      B208382
    
      
      
      
      Biosafety Level
      1 


      

      Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
      		
    
      
      
      
      Product Format
      frozen 1 kit
    
      
      
      
      Storage Conditions
      -20°C (or -70°C for long-term storage)
    
      
      
      
      Comments
      
      The Microvascular Endothelial Cell Growth Kit-VEGF contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete, low serum culture environment for microvascular endothelial cells isolated from normal human tissue. The final concentration for each component in complete endothelial growth medium is as follows:
      

        
    
      • rh VEGF: 5 ng/ml
        • 
    
      • rh EGF: 5 ng/ml
        • 
    
      • rh FGF basic: 5 ng/ml
        • 
    
      • rh IGF-1: 15 ng/ml
        • 
    
      • L-glutamine: 10 mM
        • 
    
      • Heparin sulfate: 0.75 Units/ml
        • 
    
      • Hydrocortisone: 1 µg/ml
        • 
    
      • Ascorbic acid: 50 µg/ml
        • 
    
      • Fetal bovine serum: 5% 
        • 

      

      		
    
      
      
      
      Complete Growth Medium
      
        
    
      1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
        2. 
    
      2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
        3. 
    
      3. Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
        4. 
    
      4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
        5. 
    
      5. Using aseptic technique, and working in a laminar flow hood or biosafety cabinet transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
        6. 
    
        
    
        Table 1. If using the Microvascular Endothelial Cell Growth Kit-BBE (ATCC® PCS-110-040), add the indicated volume for each component:  
        
    
        
    
        
        
            
        
                 
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        Component
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
      
        
      Volume
      
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        Volume
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
      
        
      Sterility Tests
      
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        Final Concentration
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
      
        
      Functional Tests
      
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        Bovine Brain Extract (BBE)
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
      
        
      C of A
      
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        1.0 mL
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
            

        

						
					
					
        
							
        
							  
							  
							    
        
							      梅經(jīng)理
							      17280875617
							      1438578920
							    
        
							    
        
							      胡經(jīng)理
							      13345964880
							      2438244627
							    
        
							    
        
							      周經(jīng)理
							      17757487661
							      1296385441
							    
        
							    
        
							      于經(jīng)理
							      18067160830
							      2088210172
							    
        
							    
        
							      沈經(jīng)理
							      19548299266
							      2662369050
							    
        
							    
        
							      李經(jīng)理
							      13626845108
							      972239479
							    
        
							    
							          
							
        
					
        
				
				
        
					
				
        
			
			
		
		
        
	
  
        
	







长春市|
缙云县|
孟州市|
镇坪县|
博白县|
兴隆县|
青河县|
施秉县|
阳高县|
永顺县|
乡宁县|
忻州市|
沁水县|
邵东县|
嘉祥县|
石首市|
上林县|
米林县|
赞皇县|
开原市|
祥云县|
南澳县|
四川省|
邵阳县|
武隆县|
文水县|
桂林市|
芜湖市|
邢台市|
天全县|
深泽县|
东阳市|
沧州市|
光山县|
荥阳市|
大田县|
临夏市|
盐津县|
宝应县|
奉节县|
虞城县|