| 產(chǎn)品名稱 |
Enterovirus A71 (EV-A71) |
| 商品貨號(hào) |
B208838 |
| Classification |
Enterovirus, Enterovirus A, Enterovirus A71 (EV-A71) |
| Agent |
Enterovirus A71 (EV-A71) |
| Strain |
BrCr |
| Applications |
Enteric Research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Derived by passage of ATCC® VR-784™ in Vero cells (ATCC® CCL-81™). This product replaces ATCC® VR-784™ and exhibits CPE when cells are infected.
Note: The sequences of ATCC® VR-784™ and ATCC® VR-1775™ have not been compared. |
| Effect on Host |
CPE in the form of cell rounding, sloughing and degeneration of the monolayer. |
| Recommended Host |
Vero (ATCC® CCL-81?™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 70-80% confluent. Remove medium, wash cell monolayer once with PBS, and inoculate with a small volume (eg. 1 mL per cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 5-9 days at 37ºC in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture. |
| Name of Depositor |
NJ Schmidt |
| Chain of Custody |
Deposited as Enterovirus type 71 |
| Source |
Stool specimen from 2-month-old male with aseptic meningitis. ® VR-784™ in Vero cells (ATCC® CCL-81™.). This product replaces ATCC® VR-784™. and exhibits CPE when cells are infected. |
| References |
Schmidt NJ, et al. An apparently new enterovirus isolated from patients with disease of the central nervous system. J. Infect. Dis. 129: 304-309, 1974. PubMed: 4361245
|
