国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pXC36a
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pXC36a
pXC36a
規(guī)格:
貨期:
編號:B209456
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pXC36a
商品貨號 B209456
Designations pXC36a
Depositors TA Patterson
Biosafety Level 1
Vector Information
Size (kb): 5.7750000953674320
Vector: pXC36a (plasmid)
Promoters: Promoter for expression lambda PL
Construction: pXC24, oligo cassette
Marker(s):ampR
Construct size (kb): 5.775000095367432
Features: initiation codon: ATG
marker(s): ampR
marker(s): tetR
promoter for expression: lambda PL
replicon: ROP copy number control
replicon: pMB1
restriction site: 3' sites HindIII...BamHI
restriction site: 5' NsiI/NdeI site
ribosome-binding site: synthetic Shine-Dalgarno
spacer sequence: AAAAAA
transcription terminator: partially deleted lambda terminator
coding sequence: 14-3-3
Applications
expression vector
in another host, produces protein
vector useful for cloning PCR products
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.2; EcoRI--6.2; HindIII--6.2.
The vector contains a 0.7 kb bovine cDNA that can be excised using the 5' and 3' cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression.
One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences.
The bovine insert or target sequences cloned into the NsiI/NdeI site are expressed as a Met-His fusion protein.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Cheng X, Patterson TA. Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res. 20: 4591-4598, 1992. PubMed: 1408761

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
梅河口市| 本溪市| 婺源县| 垫江县| 宁安市| 宁晋县| 湾仔区| 泊头市| 庄浪县| 保亭| 马鞍山市| 吉木萨尔县| 兰溪市| 天门市| 汉沽区| 锦州市| 家居| 玉山县| 恩平市| 嘉义县| 柳林县| 保靖县| 静海县| 凌云县| 加查县| 常熟市| 绍兴县| 天祝| 辽阳市| 星座| 兴和县| 肥东县| 蒲城县| 怀安县| 康马县| 无锡市| 铜陵市| 广宁县| 西丰县| 华坪县| 台山市|