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pHAF2
pHAF2
規(guī)格:
貨期:
編號(hào):B209462
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pHAF2
商品貨號(hào) B209462
Designations pHAF2
GenBank Number

J00077

Species Homo sapiens, human
Vector
Construct size (kb): 5.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 0.8999999761581421
Tissue: embryonal carcinoma
Gene product: alpha-fetoprotein( alpha-1-fetoprotein, alpha-foetoprotein, alpha-fetoglobulin) [AFP]
Alleles: B1, B2, C1, C2, D1, D2
Insert Size (kb) 0.900
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.4; HindIII--5.4; PstI--4.1, 0.9.
Insert covers about 40% of the mRNA sequence (3' end) including coding for domain 2 and all of domain 3 of the protein.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
References

Morinaga T, et al. Primary structures of human alpha-fetoprotein and its mRNA. Proc. Natl. Acad. Sci. USA 80: 4604-4608, 1983. PubMed: 6192439

Nakao K, et al. c-Ha-ras down regulates the alpha-fetoprotein gene but not the albumin gene in human hepatoma cells. Mol. Cell. Biol. 10: 1461-1469, 1990. PubMed: 1690841

Murray JC, et al. Linkage disequilibrium of ALB and AFP RFLPs predicts 5' to 3' orientation. Cytogenet. Cell Genet. 40: 708, 1985.

Morinaga T, et al. Afphafetoprotein messenger RNA in human embryonal carcinoma grown in nude mice, and cloning of its complementary DNA. Oncodev. Biol. Med. 3: 301-313, 1982. PubMed: 6182554

Murray JC, et al. RFLPs for the human alphafetoprotein (AFP), at 4q11-4q13. Nucleic Acids Res. 13: 6794, 1985. PubMed: 2414731

Barbara Weiffenbach, personal communication

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