Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 3.0, 0.8, 0.5; EcoRI--4.0, 3.3; HindIII--6.45. Codominant segregation demonstrated in 24 3-generation families for the MspI polymorphism. Enzyme(s) not detecting polymorphism: TaqI, RsaI. |