| 產(chǎn)品名稱(chēng) |
pKB663 |
| 商品貨號(hào) |
B210857 |
| Designations |
pKB663 |
| GenBank Number |
M10431
|
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
OmniGene, Inc., KC Backman, OmniGene, Inc. |
| Applications |
produces protein chorismate mutase-P-prephenate dehydrogenase |
| Vector |
Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pKB430
Intact vector size: 4.500
Type of vector: plasmid
Vector end: PvuII
Vector end: BamHI
Cloning sites: PvuII
Polylinker sites:
Other unique sites: BamHI
Construction: pBR322, lac promoter
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, ->
replicon: pMB1
promoter for expression: lac, ->
restriction site: PvuII
marker(s): tetR, <-
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: pheA
Gene name: chorismate mutase-P-prephenate dehydrogenase
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert 5' end: StuI
Insert 3' end: BglII
Insert size (kb): 2.17
Cross references: DNA Seq. Acc.: M10431
Nucleotides 1-2168 of the insert correspond to
nucleotides 576-2743 of M10431. Insert lengths(kb): 2.170000076293945 Gene product: chorismate mutase-P-prephenate dehydrogenase [pheA] Target Gene: chorismate mutase-P-prephenate dehydrogenase |
| Insert Size (kb) |
2.170 |
| Media |
ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; EcoRI/BamHI--4.0, 1.08; EcoRI/NcoI--3.5, 1.5. Excision of the insert with EcoRI+Tth111I yields a lac promoter - pheA cassette. The construct contains a weak attenuator sequence at the 5' end of the pheA coding sequence. |
| Classification |
Enterobacteriaceae, Escherichia |
| References |
Backman KC. Method of biosynthesis and cells therefor. US Patent 4,839,286 dated Jun 13 1989
Backman KC. Enzyme deregulation. US Patent 4,753,883 dated Jun 28 1988
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
