Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--2.7, 2.35; PstI--2.7, 1.3, 1.0; SphI--3.8, 1.1, 0.2. The insert contains the following restriction sites (approximate kb from the upstream end): PstI--0.14, 0.98; SphI--0.94; 1.75; EcoRV--1.27, 1.35. Includes sequences flanking the gene. |
References |
Kohl L, et al. Triose-phosphate isomerase of Leishmania mexicana mexicana. Cloning and characterization of the gene, overexpression in Escherichia coli and analysis of the protein. Eur. J. Biochem. 220: 331-338, 1994. PubMed: 8125090
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