Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--22.6, 13.5, 6.0; EcoRI/HindIII--22.6, 9.4, 5.6, 4.2, 2.3, 1.5, 0.45. Verified to contain the correct insert sequence using the following oligonucleotides (5'-3'): TGCAAGGTCGACGGTTATCGATAAGCTT and CAGCAGATCAAATTTGTCTTCTGAAGG. The predicted product size is (bp): 249. The observed product size is (bp): 260. |