| 產(chǎn)品名稱(chēng) |
pAqpZ plasmid in E. coli |
| 商品貨號(hào) |
B211572 |
| Designations |
pAqpZ plasmid in E. coli |
| GenBank Number |
U38664
|
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
P Agre |
| Applications |
ecause of the XhoI and SalI sites in the MCS 5' to the insert, either enzyme can be used to excise an intact open reading frame. |
| Vector |
Construct size (kb): 3.774 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.813 Gene product: aquaporin Z [aqpZ*] |
| Insert Size (kb) |
0.813 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/NotI--2.9, 0.84; SalI--2.9, 0.84; XhoI--2.9, 0.84. The insert contains the following restriciton sites (approximate kb from the 5'end): SnaBI--0.25; BsmI--0.39; PacI--0.69. The insert in this clone contains the complete open reading frame for the E. coli aquaporin Z [aqpZ*] gene plus 100 bp of the 3' untranslated region.After the 3' UTR, a SalI and XhoI restriction site have been added.Because of the XhoI and SalI sites in the MCS 5' to the insert, either enzyme can be used to excise an intact open reading frame. |
| Classification |
Enterobacteriaceae, Escherichia |
| References |
Calamita G, et al. Molecular cloning and characterization of AqpZ, a water channel from Escherichia coli. J. Biol. Chem. 270: 29063-29066, 1995. PubMed: 7493926
|
