Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--22.3, 16.0, 7.6, 3.3, 0.9; EcoRI/HindIII--23.0, 14.0, 4.8, 3.8, 2.3, 1.65, 0.8. Verified to contain the correct insert sequence using the following oligonucleotides (5'-3'): TTCCTCCCAGAGGCAGGACTTG and GTGGATGCGAACGTGGCTCTT. The predicted product size is (bp): 154. The observed product size is (bp): 154. |